Information on the location
Nye Laboratories was the laboratory where Bill Nye discussed science lessons and performed scientific experiments on his PBS show, Bill Nye The Science Guy. It is sometimes simply known as "The Nye Lab".
Appearance in the rap battle
This was the location where Bill Nye rapped and where Carl Sagan made his cameo appearance. It was only seen in Nye's verse and occasionally during Isaac Newton's verses. After Neil deGrasse Tyson entered through Newton's blackboard, Nye appeared with him in the Cosmos during his verse.